C2272-58

CD21 (CR2, C3d Receptor, EBV Receptor)

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£1,017.00

SKU:: C2272-58
Application:: IHC-Fr
Purity:: Tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human CD21.
Clone:: 2G9
Format:: Supplied as a liquid, 0.09% sodium azide.
Extra Details:: CD21 is also known as CR2, C3d receptor and EBV receptor. Its epitope maps within 5-8 short consensus repeats (SCRs) of CD21 molecule. CD21 is expressed strongly on mature B cells, follicular dentritic cells, pharyngeal, cervical epithelial cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNa. CD21 is also known as Complement Receptor Type 2 (CR2) or Epstein-Barr virus (EBV) receptor. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen): 1:10-1:20. Immunohistochemistry (paraffin): 1:10-1:20. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Optimal working dilutions to be determined by researcher. Hybridoma: p3-NS1-Ag4-1 myeloma cells with spleen cells from Balb/c mice. Positive Control: Tonsil. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice