C2272-56E
CD21 (CR2, C3d Receptor, EBV Receptor)
Size
£863.00
- SKU:
- C2272-56E
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to human CD21 expressed in CHO cells (P20023).
- Clone:
- 11D43
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- CD21 (also EBV receptor and CR2) is a 145kD member of the RCA (receptors of complement activation) family of proteins. It is expressed on T cells, B cells, and follicular dendritic cells. On the B cell surface, it combines with the BCR and CD19 to form a B cell activating complex. Mature human CD21 is 1011aa in length. It is a type I transmembrane (TM) protein that contains a 951aa extracellular domain (ECD) (aa21-971) and a short 34aa cytoplasmic tail. The ECD exhibits fifteen 60aa SUSHI repeats. Soluble CD21 is apparently generated by cleavage near the TM domain. One potential splice variant shows a deletion of (aa 847-908), while another shows an insertion of 59aa after Lys659. The ECD of human CD21 is 71% aa identical to mouse CD21 ECD. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml Direct ELISA: 0.5-1ug/ml Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells from Balb/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice