C2272-56B
CD21 (CR2, C3d Receptor, EBV Receptor) (Biotin)
Size
£557.00
- SKU:
- C2272-56B
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human HFB-1 cell line
- Clone:
- 7H167 (BU33)
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
- Extra Details:
- Human CD21 is a receptor for complement fragments C3d, C3dg or ic3D and also EBV. CD21 is also a ligand for CD23 and plays a role in IgE synthesis. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells per tube were washed and pre-incubated with 20ul of 0.3mg/ml human IgG (to block non-specific binding). 2. Cells were then incubated for 45 minutes on ice with 80ul of C2272-56B at 5ug/ml. 3. Cells were washed twice and incubated with S7973-88P, Streptavidin(PE), after which they were washed three times fixed and analyzed using a FACS. 4. A 10.7% sub population of cells stained positive with a mean shift of 1.42 log10 fluorescent units when compared to I1904-78R2 IgG1(Biotin) Murine Negative Control at a similar concentration. 5. Binding was blocked when cells when preincubated 10 minutes with 20ul of 0.5mg/ml C2272-56A; CD21. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice