C2270-03C

CD20 (CD20 Antigen, B1, B-lymphocyte Antigen CD20, B-lymphocyte Surface Antigen B1, Bp35, LEU-16, Leukocyte Surface Antigen Leu-16, MGC3969, Membrane-spanning 4-domains Subfamily A Member 1, MS4A1, MS4A2, S7)

Size

£576.00

SKU:: C2270-03C
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Raji human Burkitt's lymphoma cell line
Clone:: MEM-97
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kD non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment. Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice