C2269-83Y
CD19 (PE)
Size
£704.00
- SKU:
- C2269-83Y
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human EB-4 Burkitt lymphoma cell line
- Clone:
- 10B913
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 15% glycerol, 0.2% BSA, 0.04% sodium azide
- Extra Details:
- Human CD19 is a B cell restricted membrane protein expressed from early stages of B cell development and associates with the antigen receptor complex. Functional Application: Antibody induces adhesion of B cells Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (10ug/ml) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80ul (10ug/ml) of C2269-83Y. 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.87 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5 mg/ml anti-CD19 antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice