C2269-83W
CD19 (B Lymphocyte Antigen CD19, B4, Differentiation Antigen CD19, Leu 12, Leu-12, MGC12802) (FITC)
Size
£557.00
- SKU:
- C2269-83W
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human EB-4 Burkitt lymphoma cell line
- Clone:
- 10B913
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Fluorescein Isothiocyanate (FITC).
- Extra Details:
- Human CD19 is a B cell restricted membrane protein expressed from early stages of B cell development and associates with the antigen receptor complex. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (10ug/ml) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Raji cells were incubated 45 minutes on ice with 80ul of C2269-83W at 1:50 (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.69 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of C2269-83U. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice