C2269-55B-ML490
CD19 (MaxLight 490)
Size
£837.00
- SKU:
- C2269-55B-ML490
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Native purified CD19 from Daudi human Burkitt lymphoma cell line
- Clone:
- 3H1080 (LT19)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. CD19 is a 95kD cell surface glycoprotein expressed upon cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: Rabbit anti-mouse IgG labeled with horseradish peroxidase (HRP): I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb xMo Rabbit anti-mouse IgG labeled PE: I1903-08L: IgG, F(ab’)2 (Rhodamine) Pab Rb xMo I1903-08M: IgG, F(ab’)2, X-Adsorbed (Rhodamine) Pab Rb xMo Recommended Negative Controls: I1904-78M: IgG1 Negative Control Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice