C2269-55B-BIOTIN
CD19 (Biotin)
Size
£837.00
- SKU:
- C2269-55B-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Native purified CD19 from Daudi human Burkitt lymphoma cell line
- Clone:
- 3H1080 (LT19)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- CD19 is a 95kD cell surface glycoprotein expressed upon cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: Rabbit anti-mouse IgG labeled with horseradish peroxidase (HRP): I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb xMo Rabbit anti-mouse IgG labeled PE: I1903-08L: IgG, F(ab’)2 (Rhodamine) Pab Rb xMo I1903-08M: IgG, F(ab’)2, X-Adsorbed (Rhodamine) Pab Rb xMo Recommended Negative Controls: I1904-78M: IgG1 Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice