C2269-53A2

CD19 (B4, CVID3) (FITC)

Size

£479.00

SKU:: C2269-53A2
Application:: Flow Cytometry
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Transfected k562 cells expressing the extracellular domain of mouse CD19.
Clone:: 11E77 (1D3/CD19)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: CD19 is a 95kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.125ug/10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.