C2269-52

CD19

Size

£632.00

SKU:: C2269-52
Purity:: Purified from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Raji cells
Clone:: 2Q1262 (FMC63, B19)
Format:: Supplied as a liquid in Tris, pH 8.0, 0.4M sodium chloride, 0.2% BSA, 0.09% sodium azide.
Extra Details:: This specificity of this antibody is equivalent to that of CD19 monoclonal antibodies, based on comparative studies, antigen recognition and positive reaction with a CD19 transfected cell line. It detects a 96kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. The antibody reacts with most B cell malignancies including chronic lymphocytic leukemia and most lymphomas, but not multiple myeloma. Applications: Suitable for use in Flow Cytometry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Indirect Immunofluorescence: 1:25 Flow Cytometry: 1:25. Dilute with isotonic buffer. Use 50ul of diluted antibody per 1 x 10e6 peripheral blood mononuclear cells (PBMC) in 100ul buffer. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.