C2269-33

CD19 (Protein Cd19, CD19 Molecule)

Size

£863.00

SKU:: C2269-33
Purity:: Purified by Protein A affinity chromatography from Hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Met1-Gly287 from rat CD19 expressed in CHO cell line.
Clone:: 12K29
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with sterile 200ul sterile PBS.
Extra Details:: CD19 (also surface antigen B4 and Leu12) is a 95-110kD member of the Immunoglobulin superfamily of molecules. It is expressed by B cells, and interacts with CD21 for the purpose of reducing the threshold of the antigen signal needed to with activate the BCR. CD19 ligation also promotes B cell: follicular dendritic cell (FDC) interaction and B cell proliferation in the FDC zone of the spleen. Mature rat CD19 is a 529aa type I transmembrane glycoprotein (aa19-547). Based on mouse, it contains a 269aa extracellular region (aa19-287) plus a 236aa cytoplasmic domain. The extracellular region possesses two C2-type Ig like domains (aa20-113, 171-271) and one utilized phosphorylation site at 225aa. The cytoplasmic domain contains five potential Tyr phosphorylation sites. There is one splice form that shows a two aa substitution after Gly489. Over aa19-287, rat CD19 shares 88% and 57% aa sequence identity with mouse and human CD19, respectively. Applications: Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using rat splenocytes Immunohistochemistry: 8-25ug/ml using perfusion fixed, frozen sections of rat spleen Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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