C2269-20C
CD18a (PE)
Size
£832.00
- SKU:
- C2269-20C
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Porcine large granular lymphocytes.
- Clone:
- 3H1065 (PNK-I)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- CD18 is a single pass type I transmembrane protein and is expressed on all leukocytes and is involved in a variety of cell functions. CD18 acts as a receptor for several ICAM molecules effecting intercellular adhesion functions, it is also involved in the recognition of a variety of extracellular substrate molecules. CD18 acts as a receptor for a number of leukotoxins produced by fungi and bacteria. Clone PNK-I is able to ameliorate the effects of these leukotoxins by blocking binding of the toxins to the CD18 receptor Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice