C2268-50E
CD17
Size
£479.00
- SKU:
- C2268-50E
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human beta-2 microglobulin associated proteins from a detergent lysate of PBL. No preservative added.
- Clone:
- Huly-m13/H018.3G-6.F5
- Format:
- Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 500 mM potassium chloride, 150mM sodium chloride.
- Extra Details:
- Antibodies to human CD17 react with lactosylceramide which is expressed on granulocytes, monocytes, platelets, and basophils (1). CD17 expression is downmodulated on activated granulocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured Nalm-6 human tumor cells were washed and preincubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD17 antibody at 20ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC; this reagent cross reacts well with Mouse IgM, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.53 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice