C2268-50A
CD17 (FITC)
Size
£557.00
- SKU:
- C2268-50A
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human beta-2 microglobulin associated proteins from a detergent lysate of PBL.
- Clone:
- 7H187
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 500mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Antibodies to human CD17 react with lactosylceramide which is expressed on granulocytes, monocytes, platelets, and basophils. CD17 expression is downmodulated on activated granulocytes. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (5ug/ml); 80ul labels 5x10e5 cultured Nalm-6 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured Nalm-6 cells were washed and incubated 45 minutes on ice with 80ul of C2268-50A at a 1:50 (5ug/ml) dilution. 2. Cells were then washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.38 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD60b antibody (C2268-50). Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice