C2268-01D
CD16,32 (APC)
Size
£512.00
- SKU:
- C2268-01D
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- VH81X Tg B cells and T220 stromal cell line
- Clone:
- 93
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with Allophycocyanin (APC).
- Extra Details:
- The lymphocyte Fc-gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc-g type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying Fc-gammaR+ cells, monoclonal antibodies to the Fc-gII/III receptor have been used to block Fc receptor binding of IgG, Fc-mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc-gR for parasitic invasion. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice