C2267-32M
CD16 (Low Affinity Immunoglobulin gamma Fc Region Receptor III, IgG Fc Receptor III, Fc-gamma RIII, FcRIII, Fcgr3)
Size
£492.00
- SKU:
- C2267-32M
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human peripheral blood lymphocytes
- Clone:
- 3G8
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct (CD16a and CD16b) forms. CD16a (FcγRIIIA) is a 50-65kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48kD glycosylphosphatidylinositol (GPI)-anchored protein. It is over 95% homologous to CD16a in extracellular domain and is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex that functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). The 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 20ul/million cells in 100ul volume Immunohistochemistry: acetone-fixed frozen tissue Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice