C2267-31N
CD16 (Fc gamma RIII alpha, NK cells) (APC)
Size
£767.00
- SKU:
- C2267-31N
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Ala31-Thr215 at the extracellular domain of mouse FcgRIII/CD16 expressed in NS0 cells.
- Clone:
- 7H68
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- Receptors that recognize the Fc portion of IgG are divided into three groups designated Fcg RI, RII, and RIII, also known respectively as CD64, CD32 and CD16. In humans, each group of receptors, is encoded by 2-3 closely related genes designated A, B, and C. FcgRI binds IgG with high affinity and functions during early immune responses. FcgRII and FcgRIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Different Fc receptors can function as activators, as inhibitors, or as decoy receptors. Applications: Suitable for use in Flow Cytometry and Direct ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells using RAW 264.7 mouse monocyte/macrophage cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice