C2267-31H

CD16 (Fc gamma RIII, NK cells) (FITC)

Size

£914.00

SKU:: C2267-31H
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Porcine peripheral blood leukocytes.
Clone:: 3H1027 (G7)
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: CD16 is a low affinity FC gamma receptor expressed by polymorphonuclear cells (PMN), peripheral blood lymphocytes (PBL) and monocytes in porcine. Clone 3H1027 has been reported to block PBL mediated antibody-dependent cell cytotoxicity (ADCC) and partially inhibit PMN-mediated ADCC. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat, 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Negative Control: I1904-78R3: IgG1 Murine Negative Control (FITC) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.