C2267-29L
CD16
Size
£479.00
- SKU:
- C2267-29L
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human polymorphonuclear leukocytes
- Clone:
- 3G8
- Format:
- Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 500 mM potassium chloride, 150mM sodium chloride. No preservative added.
- Extra Details:
- Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphtidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were incubated 45 min on ice with 80ul of anti-CD16 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 13% sub population of the cells stained positive with a mean shift of 1.54 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice