C2265-35H

CD14 (Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein) (FITC)

Size

£620.00

SKU:: C2265-35H
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 3H501 (TuK4)
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: The CD14 molecule is comprised of 365 amino acids and is anchored to the plasma membrane via a cleavable phosphoinositol linkage. CD14 is the LPS and LPS-binding protein receptor and is expressed strongly by most peripheral blood monocytes and weakly on granulocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat- 1:10; 10ul labels 10e6 cells or 100ul whole blood. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-83Y1: IgG2a Negative Control Murine (FITC) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.