C2264-14B

CD13 (Myeloid Cells, Aminopeptidase N)

Size

£550.00

SKU:: C2264-14B
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Mouse intestinal APN
Clone:: 3H1010 (R3-63)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Aminopeptidase N (APN) is a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen Immunohistochemistry (paraffin): requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized mice were fused with cells of the IR983F rat myeloma cell line. Recommended Negative Control: I1904-82D1: Rat IgG2a Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.