C2264-14B-BIOTIN
CD13 (Myeloid Cells, Aminopeptidase N) (Biotin)
Size
£837.00
- SKU:
- C2264-14B-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Mouse intestinal APN
- Clone:
- 3H1010 (R3-63)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- Aminopeptidase N (APN) is a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen Immunohistochemistry (paraffin): requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized mice were fused with cells of the IR983F rat myeloma cell line. Recommended Negative Control: I1904-82D1: Rat IgG2a Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice