C2264-03C
CD13 (Alanyl Aminopeptidase, Alanyl Membrane Aminopeptidase, Aminopeptidase M, gp150, Aminopeptidase N, ANPEP, APN, CD13 Antigen, hAPN, Lap 1, Lap1, Microsomal Aminopeptidase, Myeloid Plasma Membrane Glycoprotein CD13, p150, PEPN)
Size
£812.00
- SKU:
- C2264-03C
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Balb/c bone marrow derived macrophages.
- Clone:
- 10B697 (ER-BMDM1)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- The murine homolog of human CD13 is a 160kD cell surface antigen with aminopeptidase N activity. In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13. Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Y3-Ag1.2.3 rat myeloma cells with spleen cells from immunized rats. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice