C2264-03C-HRP
CD13 (Alanyl Aminopeptidase, Alanyl Membrane Aminopeptidase, Aminopeptidase M, gp150, Aminopeptidase N, ANPEP, APN, CD13 Antigen, hAPN, Lap 1, Lap1, Microsomal Aminopeptidase, Myeloid Plasma Membrane Glycoprotein CD13, p150, PEPN) (HRP)
Size
£1,081.00
- SKU:
- C2264-03C-HRP
- Application:
- IHC-Fr
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Balb/c bone marrow derived macrophages.
- Clone:
- 10B697 (ER-BMDM1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- The murine homolog of human CD13 is a 160kD cell surface antigen with aminopeptidase N activity. In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13. Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Y3-Ag1.2.3 rat myeloma cells with spleen cells from immunized rats. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice