C2264-03

CD13 (Alanyl Aminopeptidase, Alanyl Membrane Aminopeptidase, Aminopeptidase M, gp150, Aminopeptidase N, ANPEP, APN, CD13 Antigen, hAPN, Lap 1, Lap1, Microsomal Aminopeptidase, Myeloid Plasma Membrane Glycoprotein CD13, p150, PEPN)

Size

£576.00

SKU:: C2264-03
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Cells from cattle intestine.
Clone:: CC81
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Bovine CD13 is a 150kD type II membrane protein shown to be a metallopeptidase in humans. In cattle the antigen recognised is primarily expressed on enterocytes and cells with a dendritic morphology in the small intestine. It also defines a subpopulation of dendritic cells in afferent lymph that are CC81 Ag +ve and SIRPalpha -ve, which show differences in their capacities to stimulate T cells and cytokine synthesis compared to the CC81 Ag –ve SIRPalpha +ve dendritic cells. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:100-1:1000; 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): 1:25-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.