C2264-02C
CD13 (Alanyl Aminopeptidase, Aminopeptidase M, Aminopeptidase N, AP-M, APN, AP-N, CD13, gp150, GP150, hAPN, LAP1, Microsomal Aminopeptidase, Myeloid Plasma Membrane Glycoprotein CD13, p150, P150, PEPN)
Size
£479.00
- SKU:
- C2264-02C
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human osteosarcoma tissue
- Clone:
- 22A5
- Format:
- Supplied as a liquid in PBS, pH 7.5. No preservatives added.
- Extra Details:
- Human CD13 is a zinc-binding aminopeptidase-N enzyme expressed on the surface of myeloid cells. IL-4 will upregulate expression of CD13 which may play an anti-inflammatory role. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed paraffin sections Flow Cytometry: 10ug/ml labels 5x10e5 ficoll prepared human Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured ficoll prepared human peripheral blood mononuclear cells were washed and preincubated 5min with 20ul human IgG (to block non-specific binding) after which they were incubated 45 min on ice with 80ul of C2264-02C at 10ug/ml. 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3. A net 5% subpopulation of the cells stained positive with a mean shift of 1.53 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice