CD13 (Myeloid Cells, Aminopeptidase N)

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£869.00

SKU:: C2264-01H
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Lys69-Lys967 from human CD13 expressed in NSO cells.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: The CD13 antigen is present on most cells of myeloid origin, including granulocytes and monocytes in normal peripheral blood. CD13 is not expressed on B-cells, T-cells, platelets or erythrocytes. Expression of this antigen is greater on monocytes than on granulocytes. Applications: Suitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in total reaction volume ≤200ul. Tested for use using U937 cells. Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human CD13 is ~0.3ng/well. Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human CD13 is ~5ng/lane under non-reducing and reducing conditions. Immunoprecipitation: Precipitates CD13 from conditioned media of transfected NSO cells. KO Validated: Aminopeptidase N/CD13 is specifically detected in U937 human histiocytic lymphoma parental cell line but is not detectable in Aminopeptidase N/CD13 knockout U937 human histiocytic lymphoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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