C2262-57R

CD11c (Monocyte, Macrophage, Integrin alphaX) (PE)

Size

£730.00

SKU:: C2262-57R
Application:: Flow Cytometry
Purity:: Purified from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: BU15
Format:: Supplied as a lyophilized powder from PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
Extra Details:: Integrin alphax subunit (ITGAX) is a ~150kD glycoprotein also known as CD11 antigen-like family member C or Leu M5, CD11c is expressed by macrophages, monocytes, NK cells and most dendritic cells (Kohrgruber et al. 1999). CD11c is also expressed at a lower level by granulocytes. CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b (Sadhu et al. 2007) and Junctional adhesion molecule-like (Bazzoni et al. 2011). CD11c is involved in cell-cell interactions during the inflammatory process and is important for monocyte adhesion and chemotaxis. CD11c also acts as a signalling receptor for polysaccharide (Inqualls et al. 1995) CD11c is expressed by hairy cell leukemia cells (Goodman et al. 2003; Nicolaou et al. 2003). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat, use 10ul to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice