C2262-55B
CD11c (Monocyte/Macrophage, Integrin alphaX) (Biotin)
Size
£557.00
- SKU:
- C2262-55B
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human rheumatoid synovial fluid cells/monocytes
- Clone:
- 7H115
- Format:
- Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.
- Extra Details:
- Human CD11c (alpha X integrin) complexes with CD18 (beta2 integrin) to form the complement receptor type 4 (CR4) heterodimer which binds to fibrinogen and is involved with monocyte/granulocyte adhesion during inflammatory responses. CD11c expression is restricted to leukocytes mainly of myeloid lineage with highest expression on macrophages. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry protocol: 1. Five x 10e5 ficoll prepared human peripheral blood mononuclear cells were washed preincubated ~10 minutes with 20ul human IgG at 250ug/ml (to block non specific binding). 2. Cells were incubated 45 minutes on ice with 80ul of C2262-55B at 5ug/ml. 3. Cells were washed twice and incubated with Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. 4. A 16.5% sub population of the cells stained positive with a mean shift of 1.60 log 10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 5. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml C2262-55A. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice