C2262-52F3
CD11a (Integrin Alpha L, ITGAL, Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain, LFA1A, Ly15, Ly21, Lymphocyte Function Associated Antigen Type 1 alpha, p180)
Size
£800.00
- SKU:
- C2262-52F3
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to Fibronectin purified human monocytes.
- Clone:
- 11n28
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Integrin subunit aL/CD11a is a 180kD type I TM glycoprotein that interacts only with Integrin b2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin aL contains a 1064aa extracellular domain (ECD), a 20aa TM sequence and a 58aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin aL ECD share 74aa identity. A second isoform has a 53aa insert in the ECD. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice