C2262-43C
CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor) (Biotin)
Size
£442.00
- SKU:
- C2262-43C
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes
- Clone:
- M1/70
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
- Extra Details:
- CD11b, the integrin αM subunit, combines with CD18, the integrin β2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells. Applicatons: Suitable for use in Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: ≤1ug/1x10e6 cells. Optimal dilution determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice