C2262-41B
CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor) (FITC)
Size
£620.00
- SKU:
- C2262-41B
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- T cell enriched splenocytes from B10 mice.
- Clone:
- 3H977 (M1/70.15)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- CD11b, also known as the alpha-M integrin chain, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: neat, 1:10, use 10ul of suggested dilution to label 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: NS1/1.Ag4.1 myeloma cells with spleen cells from DA rats Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice