C2262-40U
CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor) (Biotin)
Size
£633.00
- SKU:
- C2262-40U
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat peritoneal macrophages
- Clone:
- 3H953 (OX-42)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 1% BSA. Labeled with Biotin.
- Extra Details:
- CD11b is the receptor for the iC3b component of complement. It is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and ~35% of alveolar macrophages. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10; Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line. Recommended Secondary Reagents: S7973-78: Streptavidin (PE) S7973-96A: Streptavidin (FITC) S7973-96B: Streptavidin (FITC) S7973-97A: Streptavidin (HRP) S7973-97B: Streptavidin (HRP) Recommended Negative Control: I1904-83W1: IgG2a, Murine Negative Control (for rat tissue) (Biotin) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice