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C2262-40J-ML650

CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor) (MaxLight 650)

Size

£819.00

SKU:
C2262-40J-ML650
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Rat
Immunogen:
T cell enriched splenocytes from B10 mice.
Clone:
3H977 (M1/70.15)
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Extra Details:
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Recommended Secondary Reagents: Goat Anti Rat IgG: HRP (mouse absorbed), F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed) Fusion Partners: Spleen cells from immunized DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line. Recommended Negative Controls: Rat IgG2b Negative Control Applications: Flow Cytometry: 1:50-1:200 Immunohistochemistry: Frozen, Paraffin Immunoprecipitation Optimal working dilutions to be determined by researcher. Immunohistochemistry: #This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice