C2262-39P
CD11b (CD11 antigen-like family member B, CD11B, Cell surface glycoprotein MAC-1 subunit alpha, CR3A, CR-3 alpha chain, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC-1, MAC1A, MGC117044, MO1A, Neutrophil adherence receptor, SLEB6)
Size
£479.00
- SKU:
- C2262-39P
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human Monocytes
- Clone:
- ICRF44
- Format:
- Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride. No preservative added.
- Extra Details:
- Human CD11b (aM integrin) complexes with CD18 (B2 integrin) to form the complement receptor type 3 (CR3) heterodimer which binds to ICAM-1, fibrinogen, C3b, and coagulation factor X. CD11b expression is restricted to leukocytes, mainly to myeloid and NK cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Briefly, 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD11b antibody at a concentration of 20ug/ml. Cells were washed twice and incubated with 50ul of with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 15% sub population of the cells stained positive with a mean shift of 1.10 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice