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C2262-36M

CD11b (Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor) (FITC)

Size

£934.00

SKU:
C2262-36M
Application:
Flow Cytometry
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Clone:
10B594 (CC126)
Format:
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:
CD11b is also known as CD11b/CD18, Mac-1, alphaMbeta2 integrin, Cr3, MO-1, C3bi Receptor). CD11b is a marcophage-granulocyte specific antigen. Mac-1 macrophage differentiation antigen is associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1). CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and <1% of thymocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10; 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Excitation: 490nm Emission: 520nm Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.  Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice