C2262-35L

CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor) (APC)

Size

£416.00

SKU:: C2262-35L
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: Peritoneal macrophages from C57 B1/6 x DBA/2 F1 hybrid mice
Clone:: 3A33
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with APC.
Extra Details:: CD11b (integrin alphaM subunit) combines with CD18 (integrin beta2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54).2-6 Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities.2, 7-10 In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process,4, 11 Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.12 Applications: • Flow cytometry 1 • Immunohistochemistry (frozen sections) 13 • Immunoprecipitation 1 Recommended Dilution: Flow Cytometry: ≤ 0.3ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.