C2262-27E

CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL) (PE)

Size

£448.00

SKU:: C2262-27E
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: Whole BW5147 cells
Clone:: I21/7
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
Extra Details:: Leukocyte Function-associated Antigen-1 (LFA-1) is a member of the integrin family of cell adhesion molecules (1, 2). The antigen is expressed on thymocytes, T and B lymphocytes, and on non-lymphoid cells in the bone marrow (2). CD11a appears to be an essential component for T-cell activation by antigen-presenting cells and is important for cell-mediated killing. Co-stimulatory signaling by LFA-1 is achieved by pairing with its ligand, CD54/ICAM-1 (1, 2). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.2ug/10e6 cells Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.