C2262-27D

CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL) (Biotin)

Size

£499.00

SKU:: C2262-27D
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Whole BW5147 cells
Clone:: I21/7
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: Leukocyte Function-associated Antigen-1 (LFA-1) is a member of the integrin family of cell adhesion molecules. The antigen is expressed on thymocytes, T and B lymphocytes, and on non-lymphoid cells in the bone marrow. CD11a appears to be an essential component for T-cell activation by antigen-presenting cells and is important for cell-mediated killing. Co-stimulatory signaling by LFA-1 is achieved by pairing with its ligand, CD54/ICAM-1. Applications: Suitable for use in Flow Cytometry. Though not tested, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation reported in literature. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.