C2262-06P

CD11a (Integrin Alpha L, ITGAL, Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain, LFA1A, Ly15, Ly21, Lymphocyte Function Associated Antigen Type 1 alpha, p180) (FITC)

Size

£914.00

SKU:: C2262-06P
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Porcine alveolar macrophages.
Clone:: BL1H8
Format:: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: Mouse anti Pig CD11a, clone BL1H8 recognizes porcine CD11a, binding to a conformational epitope on the alpha chain of LFA-1 (CD11a/CD18), expressed on a range of cells including peripheral blood lymphocytes, monocytes, granulocytes and alveolar macrophages. Clone BL1H8 immunoprecipitates two bands of 170kD (CD11a) and 95 kDa (CD18) from porcine alveolar lysates, pre-clearance of the lysates completely removed the 170kD band, strongly suggesting the epitope for this antibody is located on the CD11a chain, although some involvement of residues on the LFA1 beta chain could not be formally ruled out (Alvarez et al. 2000 ). BL1H8 was clustered as porcine CD11a at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). Mouse anti pig CD11a, clone BL1H8 is reported to inhibit Con-A driven T-cell proliferation and the mixed lymphocyte reaction (Alvarez et al. 2000 ). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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