C2260-08A
CD9 (5H9 Antigen, BA2, BTCC-1, CD9 Antigen, Cell Growth-Inhibiting Gene 2 Protein, DRAP-27, FLJ99568, GIG2, Leukocyte Antigen MIC3, MIC3, Motility-Related Protein, MRP-1, p24, P24, Tetraspanin-29, TSPAN29, Tspan-29, TSPAN-29)
Size
£479.00
- SKU:
- C2260-08A
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Membrane antigen preparation of NALM-6 (human pre-B leukemia cell line)
- Clone:
- SN4/C3-3A2
- Format:
- Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride. No preservative added.
- Extra Details:
- Human CD9 is strongly expressed on platelets, on early B cells and on activated T cells. CD9 is a member of the tetraspan family crossing the membrane four times. Both the N-terminal and C-terminal regions are on the cytoplasmic side of the plasma membrane. Recent studies indicate CD9 may be involved with calcium mobilization. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured Nalm-6 cells were washed and incubated 45 min on ice with 80ul of anti-CD9 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice