C2260-06E
CD9 (BA2, DRAP27, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP1, P24, Tetraspanin 29, Tspan29) (PE)
Size
£735.00
- SKU:
- C2260-06E
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Membrane antigen preparation of NALM-6 (human pre-B leukemia cell line)
- Clone:
- 7H176
- Format:
- Supplied as a liquid in PBS, pH 7.5, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with PE.
- Extra Details:
- Human CD9 is strongly expressed on platelets, on early B cells and on activated T cells. CD9 is a member of the tetraspan family crossing the membrane four times. Both the N-terminal and C-terminal regions are on the cytoplasmic side of the plasma membrane. Recent studies indicate CD9 may be involved with calcium mobilization. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Cultured Nalm-6 human tumor cells were washed two times. 2. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of C2260-06E at 1:50 dilution (10ug/ml). 3. Washed three times, fixed and analyzed by FACS. Cells stained with a mean shift of 2.64 log10 fluorescent units when compared to a Mouse IgG1/PE negative control at a similar concentration. Binding was 99% blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of C2260-06B. A565/A280 Ratio: 3.16 Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
- Shipping Conditions:
- Blue Ice