C2260-06C

CD9 (BA2, DRAP27, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP1, P24, Tetraspanin 29, Tspan29) (Biotin)

Size

£607.00

SKU:: C2260-06C
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Membrane antigen preparation of NALM-6 (human pre-B leukemia cell line).
Clone:: 7H176
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Extra Details:: Human CD9 is strongly expressed on platelets, on early B cells and on activated T cells. CD9 is a member of the tetraspan family crossing the membrane four times. Both the N-terminal and C-terminal regions are on the cytoplasmic side of the plasma membrane. Recent studies indicate CD9 may be involved with calcium mobilization. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml; 80ul labels 5x10e5 cultured Nalm-6 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Nalm-6 cells were washed and incubated 45 minutes on ice with 80ul of C2260-06C at 5ug/ml. 2. Cells were then washed twice and incubated 45 minutes with S7973-88P: Streptavidin (PE), after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 2.7 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 4. Binding was blocked when pre incubated for 10 minutes with a 10X excess of C2260-06B. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap..

Shipping Conditions:: Blue Ice