C2260-06B
CD9 (BA2, DRAP27, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP1, P24, Tetraspanin 29, Tspan29)
Size
£479.00
- SKU:
- C2260-06B
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Membrane antigen preparation of NALM-6 (human pre-B leukemia cell line)
- Clone:
- 7H176
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate
- Extra Details:
- Human CD9 is strongly expressed on platelets, on early B cells and on activated T cells. CD9 is a member of the tetraspan family crossing the membrane four times. Both the N-terminal and C-terminal regions are on the cytoplasmic side of the plasma membrane. Recent studies indicate CD9 may be involved with calcium mobilization. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml labels 5x10e5 cultured Nalm-6 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Nalm-6 cells were washed and incubated 45 minutes on ice with 80ul of C2260-06B at 10ug/ml. 2. Cells were washed twice and incubated with secondary reagent IgG (FITC) goat anti-mouse after which they were washed three times, fixed and analyzed using FACS. 3. Cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice