C2259-28D
CD8a (Ly2, T cell Co-receptor, Leu2, T8) (PE)
Size
£320.00
- SKU:
- C2259-28D
- Purity:
- Purified
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Mouse spleen cells or thymocyte membranes
- Clone:
- 53-6.7
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8alpha/Lyt-2) and a beta chain (CD8beta/Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+beta- T cells mature extrathymically, while development of the CD8alpha+beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: ≤0.2ug/10e6 cells Immunohistochemistry: Acetone-fixed frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice