C2259-28A

CD8a (Ly2, T cell Co-receptor, Leu2, T8)

Size

£364.00

SKU:: C2259-28A
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Clone:: 53-6.7
Format:: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Extra Details:: In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8alpha/Lyt-2) and a beta chain (CD8beta/Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8alpha without CD8beta. It has been suggested that CD8+beta- T cells mature extrathymically, while development of the CD8alpha+beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. 1-8 Applications: • Flow cytometry 8, 9 • Immunohistochemistry (acetone-fixed frozen sections) 10 • Immunoprecipitation 8 • In vivo and in vitro depletion of CD8a+ cells 1, 4, 5 • Fractionation of CD8a+ T cells 10 Recommended Dilution: Flow Cytometry: ≤ 2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice