C2259-27L
CD8a (Ly2, T cell Co-receptor, Leu2, T8) (PE)
Size
£1,055.00
- SKU:
- C2259-27L
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Porcine mesenteric lymphocytes.
- Clone:
- MIL-12
- Format:
- Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- Clone MIl12 was clustered at the Third International Swine CD Workshop (Haverson et al. 2001). Clone MIL12 was determined to bind to the CD8a epitope on the alpha chain based on its staining pattern on T lymphocytes and on its ability to block binding of the previously characterized CD8a antibody clone 76-2-11 to T lymphocytes (Saalmuller et al.2001). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 10ul labels 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Hybridoma: P3-X63-Ag.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice