C2259-26-BIOTIN

CD8a (Ly2, T cell Co-receptor, Leu2, T8) (Biotin)

Size

£837.00

SKU:: C2259-26-BIOTIN
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: T cell clone, C6.
Clone:: 3H870 (KT15)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in 100ul. Immunohistochemistry: Frozen. Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: Goat Anti Rat IgG: HRP (mouse absorbed), F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed) Recommended Negative Controls: I1904-82D1: Rat IgG2a Negative Control Fusion Partners: Spleen cells from immunized SD rats were fused with cells of the NSO mouse myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.