C2259-22G
CD8a (T-cell Surface Glycoprotein CD8 alpha Chain, T-cell Surface Glycoprotein Lyt-2, Lyt-2, Lyt2, CD8 Antigen, alpha Chain, BB154331, Ly-B)
Size
£294.00
- SKU:
- C2259-22G
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Concanavalin A-stimulated BALB/c splenic T cells
- Clone:
- 5H10-1
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked a and b chains that form the a(CD8a)/b(CD8b) heterodimer and a/a homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 a/b heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR a/b T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 a/a homodimer is expressed on a subset of g/d TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume Immunohistochemistry: Acetone-fixed frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice