C2259-12B

CD8 (CD 8, CD8 Antigen alpha Polypeptide, Leu2 T Lymphocyte Antigen, MAL, OKT8 T Cell Antigen, p32, T Cell Antigen Leu2, T Cell Surface Glycoprotein CD8 alpha Chain)

Size

£594.00

SKU:: C2259-12B
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Sheep efferent lymphocytes
Clone:: 38.65
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Extra Details:: CD8 cell surface antigen is expressed by the cytotoxic/suppressor subset of T lymphocytes. Under reducing conditions, the antigens immunoprecipitated by C2259-12B migrate at ~33kD and ~36kD. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: NS-1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.